The effect of plant growth regulators and type of iron chelate on rooting and rooting of three cultivars of cut rose

In vitro culture method, in addition to being a suitable method for propagating commercial red flower varieties with very high uniformity and in a short time, is now considered a desirable path to improvement through genetic manipulation. Tissue culture buds of three commercial rose cut branches were evaluated for propagation and multiplication in combination with four growth regulators: BA (4.44-0.1-59 μM), NAA (21.0-0.27-0.54 μM), IBA (14.0-0.14 μM), and GA3 (29.0-0.29 μM). The MS base culture medium contained FeEDTA or FeEDDHA iron chelate. For root induction in the studied cultivars, a combined treatment including 9.9 micromolar IBA and 7.5 micromolar IAA was applied. The proliferation rate in the treatment combinations varied depending on the cultivar. The treatment of 1.59 micromolar BA and 14.0 micromolar IBA had the highest proliferation and growth percentage in all cultivars. The rooting percentage, average number of roots and root length showed significant differences depending on the cultivar. The type of iron chelate was effective on the average number of shoots produced, but had no significant effect on other traits studied in the propagation and rooting treatments.